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Once CCS reads are sorted, the pipeline uses Bowtie2's default alignment (end-to-end read alignment) to align reads to the corresponding reference sequence and outputs a set of alignments in SAM format.
In line with this, the next step tries to group together similar potential candidates from different sequences and form a set of alignments.
For a fixed parameter setting (100-bp or 400-bp mean read length), we created a set of alignments that each contained three simulated samples of metagenomic reads and the simulated reference database.
To investigate whether these biases influenced our analyses, we generated a set of alignments by progressively excluding fast-evolving positions from the original 157-protein data set and subsequently subjected the resultant data sets to the rapid ML bootstrap analysis.
One simple way of assessing the dependence of codon sites in a set of alignments is to measure the relationship between the PhyloCSF log-likelihood ratio, Λ, and the number of sites in each alignment, n.
The log likelihoods of the null hypothesis and the unconstrained tree resulted -11327.01 and -11264.55, respectively and their ratio (delta = 124.9270) was compared with the delta distribution in a set of alignments of simulated sequences evolved in silico using the unconstrained tree as guidance.
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We compute the following sum-of-pairs LCB anchoring (SP anchoring) objective function to select a set of
The input to MAPGAPS is a set of alignment profiles, a consensus sequence for each profile, and a manually-curated template alignment of the consensus sequences.
Thus, it is critical to be able to quickly and efficiently identify a set of alignment blocks that overlap a given set of genomic intervals.
We also propose a stochastic parallel refinement method that increases significantly the efficiency for the simultaneous refinement of a set of alignment candidates.
MultiZ produces a set of alignment blocks, where each block contains (presumably) orthologous regions from a subset or all the 44 species.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com