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A serial transfer of 50 mg of cells (dry weight) into fresh medium was performed every 24 h.
We experimentally manipulated host life-span in a serial transfer experiment, by killing hosts at two time points.
A study by Atsumi et al. isolated an isobutanol tolerant strain of E. coli through a serial transfer experiment [ 9].
The environment consisted of a serial transfer regime in which the populations were diluted each day into a glucose medium, supporting exponential growth for a limited time.
To test these predictions, we performed a serial transfer experiment, using the protozoan Paramecium caudatum and its bacterial parasite Holospora undulata.
During a serial transfer experiment, base pair mismatches generate "recombinants" such as a ligase composed of A5 and B3 (denoted by E5,3).
Similar(51)
Fitnesses were calculated from titers during a continuous serial transfer, 1 and 2 h after phage were added to the first culture to allow attainment of an approximate stable age distribution of infections.
We report what is to our knowledge the first evidence of bacteria phage coevolution involving the bacterium P. aeruginosa, although we only investigated this phenomenon for a single serial transfer.
To this end, an in vitro serial transfer experiment was conducted in which a colicinogenic strain (CVE2) and its plasmidless counterpart (REL606) were evolved for 253 generations.
Twelve lines that previously evolved for 20,000 generations in a benign environment, consisting of serial transfer in a minimal glucose medium at constant 37°C, all became more susceptible to freeze-thaw mortality than was their ancestor.
Two nutrient environments were used for the serial transfer experiment: a simple medium -with glucose as sole carbon source- and a complex medium.
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