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Figure 7I shows immunolabeling with PHF-1 in a sequential section.
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The largest radius of all sequential sections was denoted as the radius of the pellet, rmax.
However, observation of the sequential section series, and of the reconstructed surfaces clearly supported the conclusions of [14].
(A) Sequential cross-sections taken at ∼9.5 10.0 cm from the root tip of a nodal root of B. humidicola (CIAT 6707) grown under waterlogged conditions and (B) emergence of a lateral root from a nodal root (CIAT 16888) grown under waterlogged conditions.
Quantitative analysis was done in a total of 6 sequential sections that were about 0.16 mm apart from each other and which were collected from the central portion of the cord (sagittal) representing about 50 60% of the lesion site.
For morphometry, the epicenter of the lesion site in each of the cords was quantitatively analyzed by measuring the area of the spared tissue in a total of 6 sequential sections that were about 0.16 mm apart from each other and which were collected from the central portion of the cord representing about 50 60% of the lesion site.
Moreover, PCNA and Ki-67 are used to determinate the proliferating activity of cancer cells [ 15- 17]. Figure 5 shows an immunohistochemical analysis of sequential sections of the same piece of liver tissue in which the expression of PCNA and Ki-67 proteins in GST-p-positive preneoplastic lesions can be seen.
A short series of ultrathin (60 to 80 nm) sections containing fat body were cut from each block with an ultramicrotome (Reichert-Jung) and sequential sections were collected on mesh and formvar-coated slot grids.
Fluorescence signals were analyzed by conventional fluorescence or by scanning cells in series of 0.5 μm sequential sections with an ApoTome System (Zeiss, Oberkochen, Germany) connected with an Axiovert 200 inverted microscope (Zeiss); image analysis was then performed by the Axiovision software (Zeiss).
Figure 3 A-B and D-E depict pairs of sequential sections.
Ten cases of DCIS were identified and sequential sections cut from a formalin-fixed paraffin-embedded sample and stained for GRP78, XBP1 and the marker of hypoxia HIF-1 α.
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