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The Genome Sequencer FLX (GS FLX) is a sequencing system generating large amounts of sequence data through massively parallel pyrosequencing [2], [3], [4].
After assessing the quality of capture libraries using a bioanalyzer (2100; Agilent Technologies), the captured library was sequenced using a sequencing system (Hi-Seq 2000; Illumina) in accordance with the manufacturer's protocol.
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Sequencing analyses were performed using a fluorescent-tagged dideoxy chain termination method with a 3130XL-DNA systemcing system (Applied Biosystem).
The data were processed using a peptide sequencing system.
Survey sequencing [ 19] demonstrated the robustness of sequence assembly using the 454 GS20 (100 bp average read length) by comparing sequences with those obtained with a Sanger sequencing system.
Analysis of the library was performed using a SOLiD sequencing system (Applied Biosystems, USA) for the whole transcriptome library, generating millions of short reads of sequence.
Fragments were separated and detected with an ABI3100 sequencing system.
The sequencing reactions were analysed in an automatic sequencing system (ABI 3730XL DNA-Analyzer, Applied Biosystems) with the POP-7 system.
Clones were sequenced in both directions using T7 and T3 primers using an ABI sequencing system (Applied Biosystems).
PCR products were cloned into pGEM-T easy (Promega, Tokyo, Japan), and >20 clones were sequenced on an Applied Bioscience sequencing system (ABI PRISM 3100, Applied Bioscience LifeScience Technologiess Japan, Tokyo, Japan).
Sequencing was performed on 8448 clones by the Centre National de Séquençage (Genoscope d' Evry, France) by the SANGER method with an A3730 sequencing system.
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