Exact(2)
The Number-Letter Switching condition involves a sequencing procedure similar to that of the classic TMT.
In this study, a sequencing procedure was used to sequence simultaneously and rapidly multiple archived samples using a single standard protocol.
Similar(58)
Sequencing of purified plasmid DNA was carried out as a cycle sequencing procedure using the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems).
Ermini et al. [24], using a mixed sequencing procedure based on PCR amplification and pyrosequencing of pooled amplification products, obtained the complete mitochondrial genome sequence of a prehistoric human mummy dating 5,350 5,100 years before present [25].
This was performed as a cycle sequencing procedure using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Darmstadt, Germany) according to the manufacturer's recommendations as well as DNA sequencers ABI Prism 377 or 3100 (Perkin-Elmer/Applied Biosystems, Foster City, CA).
The analysis of post-mortem damage in the Iceman's mitochondrial sequences produced by a mixed sequencing procedure based on PCR amplification and 454 sequencing contributes to the knowledge of nucleotide misincorporation patterns in ancient DNA sequences from frozen, freeze-dried and permafrost remains.
A standard sequencing procedure with BigDye Terminator v3.1 was used to sequence PCR amplicons.
A second sequencing procedure with new PCR products confirms each mutation.
Thus far, large resequencing projects have used a standard sequencing procedure in which gene fragments are amplified by PCR, purified and subjected individually to Sanger sequencing on both strands [ 1, 2].
The product, together with the T7 selected up primers, was then subjected to an automated DNA sequencing procedure.
The developed SNPs can then be screened either with a SNP-chip that is designed based on these results or with an RAD-tag sequencing procedure.
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