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Fig. 8 Mechanisms of sequence termination.
Positively skewed pattern size distributions are thus more resilient to premature sequence termination under linear feedback.
The sequence termination and additional filtering required will both depend on the characteristics of the dataset in question.
Further development of the sequence termination criteria could improve the proportion of segments that are identified by the algorithm.
As discussed above, different sequence termination criteria could increase the percentage of segment identities assigned by the algorithm.
As expected, the higher the variability in pattern sizes, the higher is the probability of premature sequence termination.
In order to tackle this question about the mechanisms of sequence termination, we investigate the effect of skewness (or asymmetry) in the pattern size distribution, i.e., an imbalance between bigger and smaller than average patterns.
Amplicons have been directly sequenced using BigDye 3.1 Sequence Termination kit in ABI3100 Genetic Analyzer (Applied Bio Systems, Foster City, CA, USA).
A sequence will be terminated when the termination criteria is met.
We tested the propensity of the Pol II variants to read through two additional terminators in vivo using well-characterized reporter constructs containing the poly(A -dependent CYC1 terminA -dependentCYC1nd termination-independent sequencerminandon sequence (Sthenmetz et al. 2001; Steinmetz and Brow 2003).
A sequence cassette containing 13 tandem myc epitopes (myc13) and an ADH1 termination sequence was then inserted into the BamHI site that was placed immediately before the stop codon.
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