The phrase "a sequence quantity" is not standard in written English and may cause confusion.
It could be used in a specific context related to mathematics or data analysis, but it is not commonly recognized.
Example: "In our study, we measured a sequence quantity to analyze the pattern of data over time."
Alternatives: "a sequential value" or "a series quantity".
TessArray Sequence Analysis (TSEQ) software evaluates a "C3 Score" for each RPM-Flu detector tile, as a metric of detected DNA sequence quantity and quality.
An absolute target sequence quantity can be estimated [ 16, 17].
The saturation analysis was performed to check whether the number of detected genes keep increasing with the increase of sequence quantity (total tag number), it was found that when sequence quantity reaching 2 M or above, the number of detected genes almost ceased to increase (Additional file 2: Figure S3).
These problems can be overcome by employing next-generation DNA sequencers based on massively parallel sequencing technologies, by which the cloning step is eliminated and sequence quantity is increased by orders-of-magnitude compared with that of conventional Sanger sequencers.
And also, it has shown that dilution curve flattens has sequencing quantity enough.
The redundancy for Sjc-F and Sjc-M was respectively 94.2% and 94.1% which indicated the sequencing quantity should be enough for both libraries (Table 1).
Statistical data summarizing the output sequencing quantity of the three independent runs of P30, UP30 and UP40 is given in Table 1.
The distribution of time spent on processing GSM854223 and GSM854224 datasets is presented in Table 2. Apparently, the total time required depends much on mapping procedure which is closely related to sequencing quantity.
As sequencing quantities increase, this relationship reverses, and normalized libraries will capture more full-length cDNA sequences.
Next-generation sequencing offers a lower-cost option to generate a large quantity of sequence data for nonmodel species without the use of cloning, which is restricted in New Zealand for native species such as F. excorticata.
