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In their experimental work, Moldoveanu et al. were able to induce proteolysis in several mutant sequences of calpastatin by deleting one or two specific residues (Lys176, Glu177, or both) from a sequence of wild type rat calpastatin (gi 13540322) [49].
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For the phylogenetic reconstruction, a sequence of the European wild rabbit, Oryctolagus cuniculus, complete mtDNA was added to the alignment and used as outgroup (GenBank Acc. Nr. NC_001913).
The targeting construct contained a cDNA sequence of wild-type (WT) mouse exons 18 21, with a downstream phosphoglycerine kinase (PGK) promoter driving a neomycin (Neo) selection marker.
It is demonstrated by an oligonucleotide sequence of wild-type HBV (target DNA) as a model system.
B. Ujvari et al., 2007 reports an analysis of the mitochondrial genome sequence of wild lizards captured at a boundary between two different populations of lizards, which have mitochondrial genomes marked by many sequence differences.
To examine the severe inhibition of melanized microsclerotial development and defective pathogenicity that resulted from the loss of VdGARP1 function, we introduced a copy of the VdGARP1 genomic sequence of wild type V592, including the upstream 1600-bp promoter pregionr reginto inthethe mutant vdgarp1 by ATMT.
These results indicated that the sequence of wild rice W1943 had a very high similarity with those of cultivated ssp.
In conclusion, we report the transcriptome sequence of wild halophyte rice, Porteresia.
Alignment with the reference sequence for domesticated rice was used to compare the chloroplast genome sequence of wild Oryza species in Asia and Australia [ 19].
The PCR primers (Table S2) used for the initial amplification were designed based on the mitochondrial genome sequence of wild camel (C. bactrianus ferus) from public databases.
The pcDNA6- CYP11A1 vector contains the coding DNA sequence of wild-type CYP11A1 with a 14 amino acids sequence tag (V5-tag, GKPIPNPLLGLDST) at the 3′-terminus.
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