They consist of a sequence of 21 nucleotides complementary to the miRNA.
The first is the primer binding site (PBS), located immediately downstream of the 5′-LTR, where binding of a tRNA to a sequence of 8 18 bases complementary to its 3′-end serves as the primer for the synthesis of the retrotransposon cDNA strand (Mak and Kleiman 1997).
We have analysed the data from two recent studies: First, a high throughput sequencing of complementary DNAs generating short reads 30-511 nucleotides) from S. pombe growth phase and five time points during meiosis from the Bähler lab [ 25].
Erasers are also oligonucleotides with tandem repeats of a specific sequence of the complementary antisense sequence of the miRNA [ 58, 62, 63].
To investigate whether these viral miRNAs were indeed functional, a dual-luciferase reporter vector, which had four tandem repeats of a sequence perfectly complementary to each miRNA in the 3' untranslated region (UTR) of the Renilla gene, was constructed.
miRNA sponges are transcripts that carry several in tandem copies of a sequence complementary to the miRNA of interest in their 3′UTRs (Ebert et al., 2007).
The addition of a biosensor with a sequence complementary to that of the substrate increased the amount of ligation observed.
Expressed sequence tags (ESTs) are generated by single-pass sequencing of complementary DNA (cDNA) [ 1].
These findings were confirmed by Sanger sequencing of complementary DNA with transcript-specific primers (Fig. 4).
