Copy number variation (CNV) is loosely defined as a deletion, duplication or inversion of a DNA sequence longer than one kilobase.
Sequences longer than five amino acids were also included, even if the alignment was interrupted by one or two nonmatching amino acids.
We consider also sequences longer than five amino acids because the longer the amino acid sequence, the higher the probability that two proteins are similar and antibodies may also be able to bind the native protein.
In this study, a CGI is defined as DNA sequence longer than 400 bp that fulfills two conditions: 1) the GC content is above 50% and 2) the CpG observed/expected ratio is greater than 0.6 (see Materials and Methods).
For sequences longer than 100 residues, two sequences in an alignment were considered identical if the sequential overlap and the sequence identity within the overlap were both 99% or greater.
Recovery of DNA sequences longer than a few thousand base pairs from environmental samples was very difficult until recent advances in molecular biological techniques allowed the construction of libraries in bacterial artificial chromosomes (BACs), which provided better vectors for molecular cloning.
It is difficult for GLAM2 to analyze sequences longer than a few thousand residues and it is impractical for GLAM2 to analyze sequences that are ≥ 10,000 bp [ 5].
The defence began to look vulnerable and the attack toothless as Roma put consecutive wins together on just three occasions throughout the rest of the Serie A season, with none of those sequences lasting longer than three games.
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Intriguingly, in all the 10 pairs of genes that share stretches of identical sequences longer than expected by chance, the two genes in the same pair are always on two different minichromosomes, indicating that recombination occurs only between genes on different types of minichromosomes.
Analysis of linked EFT-8m TIRs revealed a 3076-bp autonomous element relic (EFT-8) with two full-length copies and another four sequences longer than 50% of full length in the genome assembly.
