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GFP was isolated from gWIZ-GFP by PCR that added a separate spacer region and a second hexa-histadine tag.
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In a separate fabrication run, spacer chips with a thickness of 100 µm are created with 20 µm high micropillars to fit into the alignment holes.
A separate benefit of the spacer was more realistic appearance to the teeth after segmentation.
They consist of a 5′-untranslated region (UTR), which has promoter activity, two open-reading frames (ORFs) separated by a spacer, and a 3′-UTR with a poly-A tail.
During manual curation of the P. carbinolicus genome annotation, the CRISPR locus was identified as 112 repeats of the sequence 5'-GAGT TCCCCGCAGA TGCGGGGATGAACCG-3' (bases in bold predicted to form a hairpin), separated by spacer sequences of 32 bp.
The setup was tested on particles in a static solution located in a chamber made of microscope slide and a cover glass separated by a spacer.
These extensions are placed in a formal framework by Brazma et al. [ 33]. van Helden et al. consider a model of spaced dyads, where two words of length three are separated by a spacer of a fixed length [ 31].
The highest scoring sequence (e-value of 3.4 × 10−115), present in all 28 species, was a bipartite motif separated by a spacer region with low conservation (Fig 6A).
An N-terminal CGGGS linker was added as a flexible spacer separating SHe from the carrier.
The PGA precursor is an inactive prepropeptide that consists of a signal (leader) peptide and a propeptide (spacer) separating α and β-subunits.
Schematic of the device shows (a) two parallel plate electrodes separated by a spacer defining the chamber.
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