Interestingly, the introduction of a non-fused phenyl ring adjacent to the amide resulted in a stereochemically sensitive increase in activity, with the R isomer 46 demonstrating fivefold more potency that the S isomer 47.
Correspondingly, the liver responds to mTORC1 activation with a rapamycin-sensitive increase in PGC1α, a key regulator of mitochondrial biogenesis, which is normally induced under fasting conditions to facilitate glucose production.
Taken together, these data indicate that, in bovine chromaffin cells, the P-CTX-1B-induced activation of voltage-sensitive Na+ channels produces a TTX-sensitive increase in intracellular Ca2+ due to both influx of Ca2+ into cells and release of Ca2+ from internal stores.
Exposure of the cultured cells to D-Asp or THA results in a rapamycin-sensitive increase in 4E-BP phosphorylation.
When CHIP28 was expressed in Xenopus oocytes, a mercurial-sensitive increase in the water permeability of the plasma membrane was observed, demonstrating that CHIP28 was a long-sought water channel protein (Preston et al. 1992).
Of further note was our observation of a MOG-sensitive increase in expression of B2m at day 15 p.i. in CP stromal capillary tissue, and at both time-points in the choroidal epithelial tissue.
In comparison with control vector-transfected cells, which exhibited a LY294002-sensitive increase in the level of p-Akt over baseline, FAK-transfected cells had a higher p-Akt level both at baseline and after treatment with doxorubicin and were sensitive to LY294002 (Fig. 5).
In Germany, weekly vaginal pH screening using a sensitive indicator (increased vaginal pH over 4.7) has been used to indicate the need for specific microbial testing and treatment.
Indeed, incubation of untreated HUVECs with A23187 triggered an l-NNA-sensitive increase in dihydroethidium fluorescence that was abolished upon loading the cells with BH4 (Fig. 5).
The researchers found that this made the cells more sensitive, increasing the rate at which they fired to both bright and dim light stimuli.
