Using the idealized PRC, the predicted consequence (from the PRC) of a 2-hr pulse was analyzed by starting at a randomly selected time point at which the pulse strikes the PRC.
At a randomly selected time point between the 2nd and 6th second after motion onset, the target object would change its current state to the other state (e.g., open book to closed book).
For the subsequent longitudinal analysis, we centered time around the date of diabetes diagnosis for case subjects and at a randomly selected time point for noncase subjects to approximate the follow-up time distribution of case subjects (i.e., year0) (16).
Multilevel models adjusted for age and ethnicity were fitted to assess 13-year trajectories of log-transformed adiponectin preceding diabetes diagnosis or a randomly selected time point during follow-up (year0) based on 755/5,095 (case/noncase) person-examinations.
In this algorithm, EEG data from 10 minutes before until 10 minutes after the selected time point were assessed.
None of the plants sampled at heading date (HD), 12 days after anthesis (DAA) or 22 DAA, showed signs of chlorophyll degradation in the flag leaves or yellowing of the peduncles (Additional file 1: Figure S3, a-c), confirming that the selected time points represent relatively early stages of the senescence process.
Comparison within one time series was performed using a global Friedman test followed by a Wilcoxon test to compare selected time points.
As an indication of cell growth, total DNA was quantified at selected time points using a Fluorstar plate reader (B&L Systems, Maarssen, Netherlands) from a standard curve performed for each assay according to the manufacturer's instructions [15], [16].
At selected time points, a 0.5 ml sample of either actively growing or non-culturable Y. pestis was removed, washed three times with PBS, and fixed with 2% (v/v) paraformaldehyde.
