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Subcutaneous tumour xenografts were established to test TRAIL-TNTs implant performance in the tumour environment by monitoring the changes in tumour burden over a selected time course.
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For the time-series data (Spellman et al., 1998), we selected time-course gene expression data from cdc28 cell cycle arrest which consists of 17 time points.
Previously, timeboxes--specifying minimal and maximal values for a continuous range of time points--were proposed [ 4] to select time courses of interest.
Additional samples were taken for determination of the free copper ion (Cu2+) at selected time points during the course of the study (day 1, 60, and 61).
Expression analysis of six selected candidates across an early senescing time course suggests that the selected time point for the mRNAseq analysis (12 days after anthesis) provided a good snapshot of the transcriptional changes associated with the down-regulation of the GPC gene.
We selected a time-course expression profiling experiment of the transition from the dauer state to the non-dauer state and the expression changes after feeding starved L1 animals [ 26] as an example (see Additional Files 1, 2, 3, 4).
Before performing correlation analyses, a band-pass Butterworth filter between 0.033 Hz and 0.13 Hz was applied on the two selected component time courses.
We selected 12 time courses corresponding to either transcription factors or cell-cycle related genes.
Fig. 6 shows the effects of CPO and pralidoxime plasma concentrations on the percentage of active AChE in two selected patients with a similar time course of the CPF concentrations.
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In selecting these time courses for further analysis, we considered those components involving regions known to mediate brain response to pain [4] and showing a consistent signal increase (activation) coinciding with each pain stimulation block, irrespectively of the duration of the activation.
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