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The instantaneous velocity field on a selected plane in Code-A is used as the instantaneous inflow conditions for Code-B.
We also demonstrate simultaneous lateral and axial actuation on a resolution target, and observe the change of lateral resolution on a selected plane through performing 1D cross-sectional images, as an indicator of focal shift through axial actuation.
In brief, ROI in a selected plane from non-saturated GFP/FM4-64 confocal image stacks corrected for the mean background staining (see above) of each rescuing LAT-1 GFP LAT-1 GFPre analyzed using the 'Intensity correlation analysis' ImageJ plugin.
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Two images are acquired: a selective inversion image, where spins are labeled with a 180° pulse in the selected plane and a nonselective image, where the whole volume is labeled with 180°.
Clicking the XY-, XZ-, or YZ-plane buttons enables the jump to the viewpoint facing the selected plane at a right angle.
In the VTQ mode, ROIs were localized to the selected plane to evaluate an average stiffness of the whole mass.
Within this selected plane, muscle variables were measured by a single experimenter: 1) Muscle-tendon complex length was calculated as the sum of the length of GM belly and length of external tendon.
Automated segmentation and tracking of other cell types, other modalities (e.g. brightfield time-lapse microscopy) and existing or new high-throughput modalities, such as selected plane illumination microscopy, may require further algorithmic developments, and therefore proper testing and validation that could be achieved through this benchmark.
After the crash, the F.A.A. ordered visual inspections of other A300-600's, but the six selected planes are to be examined using ultrasound equipment.
(A – A′ ) Maximal projection (A ) and a selected medial plane (A′ ) of deconvolved confocal image z -stacks of HeLa SS6 cells transiently transfected with a Tac-encoding plasmid.
The experimental procedure was articulated in: (i) the imposition of a selected out-of-plane condition via the micrometer screw; (ii) the collection of LVDT data; and (iii) the starting of the surface scanning and the gathering of laser sensor outputs.
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