The 5' primers (P1 and P2) are labeled with fluorescent Cy3 and Cy5, respectively; while the 3' primer (P3) contains in addition to its primer sequence an address sequence which is complementary to a secondary address sequence located on the Sentrix BeadChip.
From the second episode onwards, a secondary opening sequence runs immediately after an initial teaser: as the Mysterons announce their latest threat against Earth, the "Mysteron rings" pass over the primary puppet cast in a number of environments to demonstrate the aliens' omnipresence.
P2 probe was used as a secondary complementary sequence which can displace target DNA and hybridize with P1 probe.
We represent a secondary structure sequence of length n as nonintersecting arcs (or chords) along a horizontal axis.
Also let S= s1 s2… s L be a secondary genomic sequence (e.g. another gene) independent from G, and let S′= s′1 s′2… s′ L be its complement.
This site could also act as a secondary slippery sequence that increases the overall amount of frameshifting, thereby increasing the relative amount of protease to compensate for reduction in protease activity in the presence of inhibitor.
The secondary sequence typing approach (gyrA/katG and pncA genes) revealed a CAS-lineage specific silent C->T mutation in codon 65 of the pncA gene.
At the RNA level, HIV has evolved TAR to copy the exact secondary sequence of ASM3 in 7SK-SL1apical.
Similar to PSS, secondary sequence is also positioned in the centre 62 REs nullifying the DC subcarrier.
One hundred eighteen unique sequences, encoding 124 proteins, were mapped to the B. cenocepacia genome (Additional file 2, Table S1); of these sequences, four were secondary sequences within an already identified gene, two were within a duplicated region on chromosome one, and one was a transposase gene with nine identical copies distributed throughout the genome.
The consensus macaque sequences were aligned and fixed divergent sites between macaque and humans were mapped onto secondary sequences.
