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The results of this registration process were then averaged to create a second template.
DNA strands extended on an initial template can be displaced becoming available to prime on a second template creating the chimeras.
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Each model was aligned both with its respective template and with a fourth template that also fits the LeuT/vSGLT/Mhp1 fold (BetP).
(3) Replication progresses for ∼475 kb, resulting in the duplication of the LMNB1, PHAX, ALDH7A1, and GRAMD3 genes, and finally a third template-switching event causes the migration of the replication fork back to the strand in the direct orientation, again mediated by a microhomology (third junction C2 I2).
Moreover, a pool of total RNA was extracted from the intestinal tube tissue of S. nudus to synthesize a second cDNA template.
Each MR image was then aligned to the reference template using a high dimensional registration algorithm based on cubic B-splines (Rueckert et al. 1999, 2003) and averaged to form a second reference template.
We prove this model by showing that 85% of chimeras (Table 1) do indeed consist of an upstream sequence that has been extended on a second, nearby template of opposite polarity generating inversions (Fig 1).
But getting to a generic first template and adding my title and text are not the hard part of the process.
Polystyrene beads are electrophoretically deposited onto a sulfonate derivatized gold substrate where a patterned photoresist layer (first template) is applied.
If identities were still below the match threshold, the target sequences were compared with a second, third or fourth template.
A second generation of template plasmids was constructed by adding sequences encoding one or two artificial transcription terminators upstream of the I-SceI site.
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