Sentence examples for a second round of selection using from inspiring English sources

For enrichment, cells are put through a second round of selection using the gene-inducing conditions followed by FAC sorting of GFP-position cells.

The MICs of selected isolates representing the spectrum of mutations obtained are shown in table 3. The single strain identified as resistant to rifampicin (MIC 32 μg/ml) but sensitive to rifabutin (strain R190, MIC<0.8 μg/ml, mutation S522L) was subjected to a second round of selection using RFB, in parallel with the parent strain (Mtb72).

The pools were denatured at 95°C, snap cooled and used to perform the second round of selection using the same procedure as described for first selection.

After a second round of selection, hygromycin resistant calli were chosen for regeneration.

This mutant was subjected to a second round of selection with rifabutin.

In addition to the bait selection strategy described above (referred to as one-round selection), we simulated the network coverage results by applying a second round of selections.

The RT-cassette, inserted at the target site by an initial recombineering step using positive selection for recombinants, is replaced with the desired sequence in a second round of recombineering using negative selection.

Negative fractions from the CD14+ and CD19+ selections were then used in a second round of positive selection for CD4+ T cells and CD8+ T cells (Miltenyi Biotec), respectively.

A second round of cDNA synthesis was performed using some or all of the first round amplified RNA as indicated.

The second round of selection was performed as previously, but using 5.0 × 10 PFU in cond. 1, and 2.5 × 10 in cond. 2 and 3.

For the second round of selection, the cells selected at the first round were incubated, and β-glycosidases in cells were expressed.