Sentence examples for a second round of purification using from inspiring English sources

A second round of purification using a 2F5 antibody affinity column was carried out for ES5.

The isolated skin-ECs were purified by a second round of purification using FITC-BS1-B4.

The isolated ECs were purified by a second round of purification using FITC-BS1-B4, and purity was determined using flow cytometry (Hida et al, 2004).

The GST-fusion proteins were then cleaved with factor Xa and submitted to a second round of purification using Benzamidine Sepharose 6B (GE Healthcare) according to the manufacturer's instructions.

A second round of purification was performed using the RNeasy kit (Qiagen, Hilden, Germany) according to manufacturer's instructions.

Samples of fractions were analyzed by SDS-PAGE and fractions containing protein were pooled for a second round of purification on a Q Sepharose column (GE Healthcare).

After a second round of purification, the recovered DNA was resuspended in Qiagen elution buffer, and pipelined into the 454 sequencing procedure.

A second round of affinity purification using a 2F5 mAb column was performed.

Samples were pooled, normalizing for the number of molecules before undergoing a final round of purification using AMPure beads.

Four days following the plaque assay, a single plaque from each virus was isolated and used for a second round of plaque purification in MDCK cells.

After ligation, two round of purification using AMPure XP beads were performed followed by 4 cycles of PCR using KAPA HiFi (Kapa Biosystems).