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In order to better understand the regulations of MaSCs during pregnancy, we also assessed the regulation of MaSCs throughout a second round of pregnancy using both flow cytometry and the BLI methods (Fig. 7).
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However, in a second round of pregnancy, this expansion was not as extensive.
Finally, quantification of the alveolar density showed that during a second round of pregnancy and lactation, c-Myc mutant glands displayed a strongly reduced alveolar area).
A second round of cDNA synthesis was performed using some or all of the first round amplified RNA as indicated.
A second round of cDNA synthesis was performed using the total amount of the amplified RNA.
A second round of ORF finding was performed by using Glimmer3 [ 38] on all the contigs.
The virus rescued in round 1 was used in a second round of transfection/rescue.
For the second round of assembly, the SNPs and indels called in the first round were introduced into the D. melanogaster reference, and this modified reference was then used for a second round of mapping.
Information from the first round of testing was used to guide a second round of compound purchase.
In some cases, one round of RACE-PCR was performed; in others, a second round of RACE-PCR with a second nested gene-specific primer was used.
Those amplicons were used as primers in a second round of PCR together with the appropriate non-mutagenic primer of the first round.
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