The phrase "a second round of pcr using" is correct and usable in written English.
It can be used in scientific or technical contexts, particularly in discussions about laboratory procedures involving polymerase chain reaction (PCR).
Example: "After analyzing the initial results, we decided to conduct a second round of pcr using a different set of primers."
Alternatives: "a subsequent PCR cycle with" or "a follow-up PCR using".
The PCR products were then subjected to a second round of PCR using the N. fuckeliana specific PCR primers Cct1 (5'-ACC CCA AAC CCT TAT TTC TG-3') and Cct2 (5'-ACG GCG TGG CCG CGC CGC TT-3') developed by Langrell (2005).
In addition, all PCR showing important differences between pre- and post-treatment allelic profiles were confirmed with a second round of PCR using microdissected material and a third PCR with non-microdissected tumour tissue.
Briefly, CYP21A2 gene was amplified by PCR in three overlapping fragments, and each mutation was screened by allele-specific PCR or PCR-RFLP in a second round of PCR using one of these previously amplified fragments as templates.
Next, 1/20th of the PCR-amplified mixtures were subjected to a second round of PCR using flanking primers to yield three fragments for the three sections.
A second round of PCR using the KAPA2G Robust HotStart Readymix (2×) was performed using the first round as template due to the possibility of low fungal DNA titre.
The reaction product was then used in a second round of PCR using the same conditions.
The products of each PCR were purified and used as templates for the second round of PCR using primers "a" and "d".
The products of each PCR were purified and used as templates for the second round of PCR using the primers a and d.
A second round of PCR was used to improve specificity and to add recognition sites for the 454 primers necessary for the emulsion PCR step preceding pyrosequencing [46].
A second round of PCR was conducted using the extracted DNA as a template.
For reactions with comparatively low yield, either PCR conditions were individually adjusted (mainly on annealing temperature; Table 1) or a second round of PCR was performed using the inner primers and the first-round PCR product as template.
