Sentence examples for a second round of minimization using from inspiring English sources

A second round of minimization using 100-mer RNA sections found 101 200 to possess only very weak EZH2-EED binding capability (no saturation up to 5 μM), whereas 201 300 retained full affinity for EZH2-EED (Kd = 157 ± 12 nM), strongly suggesting the protein binding domain resided within this final 100 nt of the HOTAIR 5′ 300-mer domain.

A second round of amplification used the first polymerase chain reaction products to specifically detect D pneumosintes.

A second round of cDNA synthesis was performed using some or all of the first round amplified RNA as indicated.

Small perturbations in translation and rotation (0.1 Å, 3°) were introduced with side chain repacking and energy minimization, followed by a second round of small perturbations in NADP position, orientation and torsions prior to a final energy minimization.

The virus rescued in round 1 was used in a second round of transfection/rescue.

For the second round of assembly, the SNPs and indels called in the first round were introduced into the D. melanogaster reference, and this modified reference was then used for a second round of mapping.

Information from the first round of testing was used to guide a second round of compound purchase.

Furthermore, we also perform an energy minimization step of the entire pMHC structure to adjust the 'MHC donor' cleft to this new epitope followed by a second round of molecular docking.

A first round of talks was postponed.

Do a third round of 30 breaths.

The second round of amplification, using random second round primers, was started with 1.0 μg aRNA in case of macrodissected samples.