The phrase "a second master of" is correct and usable in written English.
It can be used when referring to someone who has achieved mastery in a particular field or skill, in addition to another master they may already have.
Example: "After years of dedication, she became a second master of the art of painting, complementing her expertise in sculpture."
Alternatives: "a secondary expert in" or "an additional master of".
The reaction mix was incubated at 65°C for 10 minutes prior to addition of 23 µL of a second master mix containing per reaction: 10 µL of 5× First Strand Buffer, 5 µL of 0.1 M DTT, 2.5 µL of dNTP Mix, 1 µL of Superase-in RNase Inhibitor, 2 µL of SuperScript II Reverse Transcriptase (200 U) (Invitrogen), and 2.5 µL of nuclease-free water.
The reaction mix was incubated at 65°C for 10 minutes prior to addition of 23 μL of a second master mix containing per reaction: 10 μL of 5× First Strand Buffer, 5 μL of 0.1 M DTT, 2.5 μL of dNTP Mix, 1 μL of Superase-in RNase Inhibitor, 2 μL of SuperScript II Reverse Transcriptase (200 U) (Invitrogen), and 2.5 μL of nuclease-free water.
For example, a second master trainer was identified out of the pool of HC trainers and had started the master training certification process in 2011, but had not completed it as of December 2011.
Ridgely died that same year, his nephew, Charles Carnan Ridgely (1760 1829), became the second master of Hampton.
Pascal is the first master of a really modern prose style.
Tiger Woods's hopes of a fifth Masters title remain alive after the world No1 survived the threat of disqualification after taking an incorrect drop during his second round.
The first master of the house was a man named Thomas Lewen, a merchant by trade.
If not, inconsistent tee shots could fatally undermine his hopes of a fifth Masters title.
But Selby doubled back off the cushion to pot an excellent blue into the middle pocket and then added the pink to win the match and keep alive his hopes of a fourth Masters title in seven years.
Tiger Woods declared himself ready to compete for a fifth Masters title after completing 11 holes of practice at Augusta National on Monday.
