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This forms a Schiff's base that can participate in a second linkage, creating methylene bridges between amino acids that were in close proximity (∼2 Å) in the native protein.
An interdomain disulfide (CYS113 CYS254) provides a second linkage between Domain A and B. A large central cavity, formed by helices α2,α3,α4,α7, and α8 is visible within NPC1L1 NTD) (Fig 3A,C).
The five remaining markers from Mus Chr 12 form a second linkage group in Peromyscus.
We performed a second linkage mapping analysis with the Chr 10 QTL regressed out but did not find any additional QTL at the genome wide p < 0.05 level.
To overcome the potential effects of these factors, we performed a second linkage analysis taking these two factors into account as covariables in the model.
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The molecular toolbox produced for the gilthead sea bream which includes a first linkage map (LG map) based on some 200 microsatellite markers [16], EST (Expressed Sequence Tag) studies (e.g. [17], [18] as well as the construction of the first radiation hybrid map (RH map) of the species [19] has paved the way for genetics and genomic investigations of the molecular mechanism of sex reversal.
In a first linkage cycle only records with a date of death were considered for the lining.
Double coding systems provide added privacy protection by separating research data from personal identifying data with a third "linkage" database.
In the 'A' family, three linkage variants form a sub-family (A1a-A1c) while a fourth linkage is the sole member of the second sub-family (A2).
These two databases are mediated by a third "linkage" database that contains a list pairing the two numerical codes for each patient.
They suggest using anonymized versions of the data sets for a first linkage step that is capable of classifying a large portion of record pairs correctly as matches or mismatches.
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