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The phrase "a second line of" is correct and usable in written English.
It can be used when referring to an additional line or layer of something, often in contexts like writing, design, or analysis.
Example: "The poem features a second line of imagery that enhances the overall theme."
Alternatives: "an additional line of" or "a subsequent line of".
Exact(58)
Safety systems are only a second line of defense.
A second line of barriers blocked access below Houston Street to everyone but residents.
A second line of argument would result in a substantive ruling — but one with limited scope.
Then there is a second line of logic: "Buy 'em when the news is ugliest".
She will also wear a second line of clothing, called the Tournament Series Collection.
A second line of division in memory lay between east and west.
A second line of traditional jet dryers follows the modules, finishing the drying process.
A second line of defence that took effect immediately would therefore be desirable.
Once flu viruses get into cells, the body resorts to a second line of defense.
B7 THE VACCINE -- Health officials have taken a step toward opening a second line of defense against anthrax.
Similar(1)
A second line-of-argumentation bases on the principles of ecosystem analysis and the theory of ecological orientation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com