The phrase "a second copy in" is correct and usable in written English.
It can be used when referring to an additional version of a document or item that is located in a specific format or medium.
Example: "Please send me a second copy in PDF format for my records."
Alternatives: "an additional copy in" or "a duplicate in".
Insertion of tagged PCNA as a second copy in this way did not cause slow growth in the absence of Elg1, nor was sensitivity to MMS significantly increased.
Note that our operational definition of a "single-copy" gene only requires that the fragment is not present as a second copy in the genome with similarity higher than 50%.
In contrast, if full-length DPB11 is expressed as a second copy in dpb11Δ C dot1Δ cells, the checkpoint defect is rescued to levels comparable to WT or dot1 strains.
To address this issue, we instead constructed strains with the POL30-3myc gene (under control of the native POL30 gene promoter) inserted as a second copy in addition to endogenous untagged POL30 gene.
It is known that Cdc48 forms homo-oligomers in the genuine ERAD (Aker et al. 2007); the presence of a second copy in the diatoms PPC offers the possibility for the formation of hetero-oligomers of both proteins sCdc48-1/2 sCdc48-1/2.
A third copy in red chalk from the Biblioteca Ambrosiana in Milan, more detailed in the coiffure, is probably an attempt to reproduce another Leonardo sketch, no longer extant.
From these nine phages we have isolated and described four hsp70 copies in tandem orientation hsp70S2-S55) and a fifth copy in inverse orientation (hsp70S1).
Their characterization in Grasses and the recent identification of a third copy in rice, maize and sorghum raises questions about PSY gene family organization in wheat.
The HPT profiles shown in Figure 4A indicated that the number of T-DNA copies present in TG5E01, TG5E02, TG5E03 and TG5E04 was, respectively, two, one, two and two, while the gfp probe also highlighted a third copy in TG5E01.
The presence of only two copies in eudicots was our starting point to explore evolution of the PSY gene family to explain the presence of a third copy in monocots.
Repeat H is particularly informative because there were already two copies of this 340 bp repeat within the Col-0 and C24 genomes, and the fragment inserted as part of the 1.8 kb chimera upstream of cox3 introduces a third copy in Col-0 (see fig. 1).
