The phrase "a second challenge with" is correct and usable in written English.
It can be used when introducing an additional difficulty or issue related to a specific topic or situation.
Example: "In addition to the first challenge, we are now facing a second challenge with the implementation of the new software."
Alternatives: "an additional challenge regarding" or "a further challenge concerning".
Moreover, after a long exposure with tumor cells, purified BMDC were unable to respond to a second challenge with TLR ligands.
Barcelona's initial appeal was rejected by the competition committee and the club lodged a second challenge with a higher appeals committee which ruled in their favour on Thursday.
However, preincubation for 10 min with Orai pharmacological inhibitors, YM58483 or Synta66, reduced the rate of Ca2+ influx observed in response to a second challenge with TNFα, to a value similar to basal (Fig. 2A middle and right).
Initial exposure of monocytes/macrophages to LPS induces hyporesponsiveness to a second challenge with LPS, a phenomenon termed LPS tolerance.
One week later, a first challenge with 0.5% Ox in acetone was applied to the right flank, followed by a second challenge with 0.5% Ox in acetone 2 days later.
Conclusions While human endotoxemia attenuates the inflammatory response of a second challenge with endotoxin with approximately 70 %[2], it does not influence the Fluenz-induced local immune response and viral shedding.
A third challenge with using health outcomes as a measure is that this often requires setting a global standard (e.g., longer life is always better, or less pain is always better).
For quantitative RNAseq analysis using Illumina HiSeq2000, a second challenge experiment was performed with S. aureus and 10 individuals were collected at 6 hr and 1, 3, 5, and 7 d after challenge.
That presented Nokia with a second challenge, says Mr Ollila.
A second challenge was printing localised intervention posters with photos of village leaders endorsing HWWS.
In both groups, a considerable fraction of the animals survived the second challenge, with 67% and 50% survival for the single and double administration groups, respectively (Fig. 6B; Table 3).
