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With a script developed in Developer XD, ISGs were automatically identified as objects inside the cell mask with an electron-dense core (variables listed in ESM Methods 1).
More specifically, for each utterance specified by an input text, DECtalk generates an output file having 18 parameters that can be mapped to the 13 parameters of the HLSyn synthesizer [18] input file through a script developed in Java by our group and called DEC2HLSyn.
Subsequent generations, GS, and evaluation were simulated using a script developed in R version 2.15.2 [ 10] (Table 1 and Figure 1).
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From each image stack, maximum intensity projections (for SR, mito-GFP and TMRM) were made by using a script developed in-house for ImageJ 1.31i public domain imaging software.
Using Strip_Sam_Duplicates, a script developed in-house (Dr. Chris Creevey, personal communication), multiple reads that aligned to the exact same position in the genome were removed due to their potential PCR bias introduced during library preparation.
From each image stack, maximum intensity projections (for Hoechst and PI) and autofocus images (for DIC) were made for each time point by using a script developed in-house for Fiji public domain imaging software (http://fiji.sc/wiki/index.php/Fiji).sc/wiki/index.php/Fiji
Following translation of the assembled contigs into the six possible reading frames with EMBOSS TranSeq (Rice et al. 2000) we investigated the virtual mussel proteins for the presence of the C-C-CC-C-C signature, allowing a spacing between cysteine residues of one to ten amino acids, using a Perl script developed in-house (available upon request to the corresponding author).
In order to tally cells with duplicated and non-duplicated origins, we processed KAMS-acquire files with a Matlab script developed in-house to obtain ordered images of individual cells (S. Hong, unpublished), which were then counted manually.
We performed image analysis using an ImageJ script developed in-house.
Using an Perl script developed in-house, Illumina reads were filtered out if the average Phred quality score was lower than 20.
P-values were then calculated for each gene on the microarray using the two-tailed Fisher's Exact test using an Microsoft Excel script developed in-house.
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