Our guiding hypothesis was that weak binding lead peptides could be readily identified from a screen of a small chemical library of unstructured short random peptide sequences.
A screen of a small molecule chemical library revealed that the Epigallocatechin-3-gallate (EGCG) could bind to RIG-I and modulate its signaling.
(A ) Screen of ∼30,000 small molecules at 10 μM against E. coli for cold sensitivity.
To validate the final HTS assay configuration, a screen of a small compound set (validation set) was run in triplicate.
EL102 was identified by Elara Pharmaceuticals as a potential chemotherapeutic agent during a screen of novel small molecule inhibitors using the NCI-60 cell line panel assessing for growth inhibition potential.
In a random screen of small molecules, Kitambi et al. recently identified novel molecules inhibiting hyaloid angiogenesis in zebrafish larvae [21].
The 427 GUS-PAD1 3′ UTR cell line was used to carry out a pilot screen of small molecules with the aim of identifying novel chemical inducers of stumpy formation.
They applied this assay in a screen of 2000 bioactive small molecules and identified 46 compounds with anticonvulsant activity.
It is also useful for correcting for some occurrences of quenching or light scattering that would be encountered with a high throughput screen of small molecules.
