Sentence examples for a samples was from inspiring English sources
The composition of the lysozyme and RNase A samples was analyzed by measuring the intensity of the protein monomer and oligomer bands using Un-Scan-it Gel 6.1 analysis software (Silk Scientific Corp., Orem, UT, USA).
Immediately after thawing and rehydration a samples was taken to determine cells concentration and viability, the rest of each sample (1.5 ml) underwent a washing process by centrifuging it for 10 minutes at 250 g after which the supernatant was discarded and the pellets were re-suspended with 1.5 ml RPMI-1640 (Stgma, St. Louise, MO, USA).
Similar location information for introns identified by alignment of RNAseq data, for the poly(A) samples, was extracted and the two datasets were compared to identify introns validated by RNAseq.
The capacity of each of these 26 dysregulated genes (23 up-regulated and 3 down-regulated) to discriminate between group B and group A samples was then tested via ROC curve analysis.
The total amount of RNA in WB-6 and Ov-A samples was approximately 10 µg.
RNA isolation from WB-6 and Ov-A samples was carried out with mirVana miRNA Isolated kit (Ambion), which increases the yield of small RNAs.
miR-29c, shown in previous sections to be down regulated in basal-like samples as compared to luminal-A samples, was found to be the most differentially expressed miRNA between the two ECM classes (TNoM p<3E-5, lower in ECM1, Table S8).
a Samples are normalized.
Then, a sample was taken and centrifuged.
As soon as a sample was handed over it was held up to the light.
