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A sample was set 273 mm upstream of the FZP in the on-focus condition.
To obtain a weighted instead of a simple average, the regression weight in a sample was set to zero when a variable was not selected [ 35].
Raw peak data were normalised, so that the sum of all the peak heights for a sample was set to 100.
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The minimum number of sequence counts in a tissue sample was set to one resulting in a total number of 2.954 UniGene IDs for which gene expression could be detected by 454-sequencing in all six samples.
Based on these findings, the cutoff value for a positive sample was set at 10 cells, which resulted in one positive donor bone marrow sample (1.2%).
The definition of a positive sample was set according to the manufacturers' instructions; for CHEKIT Q Fever >40 (weak positive >30 to <40), and for ELISA Cox > 40 for serum and individual milk samples and >30 for pooled milk samples.
The cut-off point of OD values of a positive sample was set to be at least two times higher than that of the negative samples at any dilution point.
Generally, 2 4 mg lysate was used for each IP experiment and an input sample was set aside following the pre-clear step.
Each soil sample was set into a pressure cell with a membrane filter (0.5 μm) set at the sample bottom.
Each patient sample was compared to a healthy control sample taken during the same week, which was used to determine gating for positive and negative populations: the histogram of a healthy control subject's sample was set not to cover more than 5% of the cells of the population, and this gate was copied to patient samples.
The thickness of the sample was set by a polytetrafluoroethylene stripe (10 or 30 μm).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com