In NMR a sample is dissolved and placed in a thin tube, which is spun between the poles of a powerful magnet.
As an attempt toward the materialization of these unique supramolecular polymers, a selected sample was dissolved in methylene dichloride and electrospun into micro-sized particles.
DNA from a mouthwash sample was dissolved in 40 μl TE buffer, and 2 μl was used for each PCR reaction.
For GC × GC-TOF MS analysis, a whole condensate sample was dissolved in a solution of 5% dichloromethane (DCM) in n-hexane.
A 6.8 mg sample was dissolved in 600 μL CD2Cl2 and analyzed at 298°K.
Therefore, to perform the HDX at the α-C of denatonium cation, a denatonium benzoate sample was dissolved in 1 % TEA/D2O solution and incubated from 1 min to 4 h at room temperature.
In detail, a small (500 µL) sample was dissolved in 100 mL of highly pure (milli-Q) water, and the resulting solution filtered through a 0.2 µm Whatman Teflon syringe filter.
After digestion, a 10 μl RNA sample was dissolved for 30 min at the room temperature.
The purified and lyophilized sample was dissolved in a minimum amount of 50mM malonate buffer, and subjected to 12% native PAGE and 10% SDS-PAGE using a Vertical Minigel electrophoresis apparatus (V-GES, Wealtec Corporation, U.S.A) to determine sample purity and approximate mass of laccase [36].
The freeze-dried sample was dissolved in a small amount of water and centrifuged.
