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In the CALUX assay (BioDetection Systems BV), the dioxin-like activity of the compounds in a sample is assessed via in vitro activation of the Ah receptor of cultured H4IIE cells.
The presence or absence of a CNA in a given sample was assessed using the same copy number cutoffs used for the GISTIC analysis.
The clinic's catchment area was clearly defined, referral was by local family doctors and community mental health teams, and a consecutive sample was assessed, supporting the representativeness of the results.
A 10% random sample was assessed by a second investigator and compared with the assessment of the first investigator.
For both steps, a 10% random sample was assessed by a second investigator (M.W. Langendam) and compared with the assessment of the first reviewer.
On the other hand, a large patient sample was assessed within a 10-year course around their first revision surgery and the core functional outcomes were reported based on current standards.
In women who had not menstruated for 60 days or longer, a random hormonal sample was assessed.
Subsequently, a 10-μl aliquot of each sample was assessed on a 1% agarose gel, and a clear DNA smear was visible between 500 and 4,000 bp.
The RNA integrity of all samples was assessed on a BioAnalyzer; all samples had a RNA Integrity Number RINN) ≥8 (Agilent Laboratories).
> -wrap-foot> The presence of an oocyte in each FF sample was assessed immediately, and samples without an oocyte were discarded.
Before target production, the quality and quantity of each RNA sample was assessed using a 2100 BioAnalyzer and a RNA 6000 Nano LabChip Kit (Agilent Technologies, Palo Alto, CA).
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CEO of Professional Science Editing for Scientists @ prosciediting.com