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A large sample was assembled that allowed to study a wide set of variables and to explore characteristics among women separately.
A set of 5 μg fluorescent-labeled cRNA targets from each sample was assembled into a hybridisation reaction on the Mouse Oligo Microarray (Agilent Technologies) using the In Situ Hybridization Kit Plus (Agilent Technologies).
For each group, each APD sample was assembled on an aluminum plate, with a PCB attached from the back (see Figure 1).
Each sample was assembled de novo using MetaVelvet [ 70] and a kmer of 51.
This sample was assembled by empanelling two groups of students of essential analytic interest: (1) a sample chosen randomly from the 6,650 Harvard undergraduates (N = 319), and (2) a "friends" sample (N = 425) composed of individuals who were named as a friend at least once by a member of this random sample (see Supporting Information Text S1 for more details).
The study sample was assembled from lists provided by The Human Resources Departments.
Each sample was assembled in ABySS using every 5th kmer ranging from 26bp to 96bp.
Between 2.22 and 7.35 Gbp of trimmed data from each sample was assembled independently.
The dataset for each sample was assembled into contigs via the software MIRA 3 [ 105], using the default setting for the 454 platform.
Two electrodes with identical sample were assembled with a cotton cloth separator in an electrolytic solution of aq. 1 M H2SO4 solution.
The insulin-EMMIPs obtained by recognizing insulin from sample were assembled onto a magnetic glassy carbon electrode (MGCE) surface in order to form the magnetically controlled membrane under the magnetic field induction, and the membrane was peeled off from the electrode by removal of the magnet after electrochemical measurement.
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