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Besides comparative analysis, a round of database queries was also performed to integrate additional information.
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The PSSM is then submitted to a round of sequence database search, same as in PSI-BLAST.
To use all available sequence data, CTG proteins with a top database hit to a bacterial protein in UniProt were extracted for a second round of database searching against GenBank (E value of 10-20).
Related family members were identified by a second round of database searching against GenBank to ensure all available genomic data was utilized.
Following a first round of database searching and on-target recrystallization of the sample spots, MS/MS spectra were collected on selected precursors (see Additional file 3 for detailed information).
Peptide mass fingerprint (PMF) data were collected on an UltraflexIII MALDI ToF/ToF mass spectrometer (Bruker Daltonics, Bremen, Germany) and used for a first round of database searching using MASCOT 2.3 (http://www.matrixscience.com) against NCBI nr core collection and the potato genome sequencing consortium (PGSC) Solanum tuberosum group Phureja DM protein database.
A round of consolidation followed.
A round of restatements followed.
— ordered a round of shots.
A round of cards was dealt.
That brought a round of applause.
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