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Tissue was dissociated with silicone-coated Pasteur pipettes and centrifuged at 1000 rpm at 4°C for 10 min. Dissociated neurons were plated on glass coverslips pre-coated with Matrigel (BD Biosciences) at a rough density of approximately two hippocampi per 24 coverslips.
We estimated a rough density of nine individuals per 100 km2 in Tabin Wildlife Reserve.
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Based on the very rough density of nine individuals per 100 km, we assume that at least all areas smaller than 350 kmightght be too small to contain a stable population of clouded leopards (> 50 individuals).
Roughness blocks whose heights were one eighth of the hill height were arranged in a staggered pattern with a roughness density of 4.1% to model the rough conditions.
On the basis of six clouded leopard track sets we estimated a rough minimum density of clouded leopards in our small study area and extrapolated our local results to the landscape level.
Davies and Payne [ 26] provided the only previous, but very rough, density estimate of clouded leopards in Sabah.
As a rough guide, the density of air at sea level when the temperature is 15 °C is 1.225 kg/m3.
A rough estimation of the density of states is given from temperature measurements.
One can, however, obtain a rough estimate of the densities from experimental data.
Again, opposed to that the UKF approach yielded only a rough approximation of the overall density.
However, the constraints of possible orbits allowed a rough determination of Ida's density, which revealed that it is depleted of metallic minerals.
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CEO of Professional Science Editing for Scientists @ prosciediting.com