Exact(5)
Collision energy was determined using a rolling collision energy equation.
A rolling collision energy was used to promote fragmentation and the collision energy range was ∼20% higher than that used for unlabeled peptides due to iTRAQ tags.
Following each survey scan, the top two precursor ions with multiple charge states with intensities greater than 150 counts/s were selected for MS/MS acquisition using a rolling collision energy based on the ions' observed charge states and masses.
A rolling collision energy setting was used.
The MS/MS was carried out in high sensitivity mode with 100 ms accumulation time and a rolling collision energy based upon mass and charge with a spread of 20.
Similar(55)
Within each well, peaks of parent ions which were within the m/z 800∼3,000 range with intensity above 10 counts ± include/exclude list will be selected automatically for collision-induced dissociation (CID) MS/MS using argon as collision gas and a mass dependent ±5V rolling collision energy until end of probe pattern was reached, starting from the most intense peak.
IDA was carried out using parameters of Top 30 (TOFMS 250 ms accumulation time, production 60 ms, total cycle time 2.1 s); charge state 2 4 above a threshold of 200 cps; dynamic exclusion for 10 s using rolling collision energy (optimised for m/z of target ion).
A sweeping collision energy setting of 35 ± 5 eV coupled with iTRAQ adjust rolling collision energy was applied to all precursor ions for collision-induced dissociation.
Fragmentation energies were set to automatic (rolling collision energy).
The DP, DP2, and FP settings were 60, 10, and 230, respectively, and rolling collision energy was used.
Eluted peptides were analyzed by one full MS scan and four consecutive product ion scans of the four most intense peaks, using rolling Collision Energy and Dynamic Background Subtract function.
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