Exact(6)
It is of note that intra-mPOA administration of kisspeptin-10 evoked a robust release of LH, thus it can be presumed that the antagonist delivered into this region also has access to the kisspeptin receptor.
Interestingly, NK cells treated with BAT3-depleted exosomes failed to produce TNF-α and IFN-γ, whereas a robust release was observed with control exosomes (si-c) derived from control si-RNA transfected cells.
It is worth noting that we recently reported that intravenous administration of kisspeptin-10 elicits a robust release of LH, and presumably GnRH, without affecting the MUA volleys in the rat [20]; data that are seemingly inconsistent with the notion that the MUA volleys reflect neuronal discharge recorded from GnRH neurons per se.
Both cell populations respond to IL-1β, demonstrated by a robust release of CXCL8 and increased expression of COX-2 mRNA from passage one (p1) through to p10.
In our study, peripheral TNF- α caused a robust release of MCP-1 both in serum and in whole brain tissue.
Treatment with IL-1β also resulted in a robust release CXCL8 (20 000-50 000 pg/ml) in upper (ANOVA P<0.0001) and lower (ANOVA P=0.0013) segment cells from p1 through p10.
Similar(54)
In this context, the low level activity observed during the second 24 hr period a.p. in non-diapausing animals may represent a leakage activity that precedes the full blown proliferative activity that initiates around 48 hrs a.p. in response to a more robust release of hormone.
Unfortunately, due to its small size, its development as a therapeutic requires a robust controlled release system.
As indicated in the representative fura-2 calcium traces in Figure 2A, addition of UTP induces a robust calcium release from the ER into the cytosol in calcium-free media.
Under Ca2+-free conditions, a robust Ca2+ release upon addition of 8-Br N-cIDPR was observed.
Amphetamine (10 µM) induced a robust dopamine release from wild-type human DAT cells (quantified by amperometric current).
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