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The mECT can be made using cells from existing mouse models of cardiac disease, providing a robust readout of contractile performance and allowing a rapid assessment of genotype phenotype correlations and responses to therapies.
This assay provides a robust readout and might help conduct genetic and drug screening studies.
First, we employed a robust readout of neurodegeneration the degeneration of the photoreceptor neurones (rhabdomeres) (Fig. 5A and B).
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We speculated that the embryonic developmental phenotype of EWS-FLI1 transgenic embryos might therefore provide a more robust readout of the effects of EWS-FLI1 on conserved signaling pathways that normally function during development.
Compared to ratiometric- and fluorescence anisotropy-based techniques, fluorescence lifetime flow cytometry provides a direct, robust readout with less demand for controls and applications beyond the measurement of FRET.
The goal of the assay, however, was to design an assay in T-cells with a definitive and robust readout.
Especially useful for dissecting complex signal transduction pathways are reporter constructs, that give a fast and robust readout of the signals running through the pathway of interest.
Nonetheless, compared with the eye phenotypes obtained in fly models of Huntington's disease (47– 51), as an example, the tau eye phenotype provides a highly sensitive and robust readout that makes it quite useful for assessing phenotypic effects of genetic manipulations.
As described in this paper, combination of the ChIP assay with robust readout methods is extremely powerful for a variety of whole-genome analyses in order to define the functional components within mammalian genomes.
We found that an antibody combination of Pecam1 together with Cd47 or Cd40 provided the most robust readout.
Nuclear magnetic resonance spectroscopy (NMR) provides robust readouts of many metabolic parameters in one experiment.
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