The phrase "a rfp" is not correct in standard written English; it should be "an RFP" since "RFP" begins with a vowel sound.
You can use it when referring to a "Request for Proposal," which is a document issued by an organization to solicit proposals from potential vendors or service providers.
Example: "We are currently reviewing a RFP from several companies to find the best fit for our project."
Alternatives: "an RFP document" or "a request for proposals."
MiR-124a or miR-scramble was co-transfected with pTα1-RFP into F11 cells, and the changes of RFP reporter expression were examined every 30 min during 48 h. a RFP signal increased gradually in F11 cells treated with miR-124a, but not in miR-scramble treated cells.
Overexpression of Sec61β containing small epitope tags, but not a RFP tag, induces dramatic bundling of the ER and microtubule.
As in Google gave the company a RFP with quite detailed technical specifications and has begun discussions on building it.
(E) A RFP targeted to the ER lumen (ss-RFP-HDEL) was expressed in wild type (wt) or sey1Δ yeast yeast cells.
The vectors 2×35S::OsLAP6-GFP and ER-marker, a RFP that fused with the KDEL ER-retention signal and also driven by the double 35S promoter (De et al. 2011) were co-transfected into in tobacco (Nicotiana benthamiana) leaf epidermal cells, and the signals of GFP and RFP were observed as described previously (Zou et al. 2017b).
Interestingly, the interaction is inhibited when a RFP tag is added to the N-terminus of Sec61β, explaining why this activity was not seen previously (Sec61β is most commonly used in the form of a GFP/RFP fusion protein as an ER marker for live cell imaging).
Analogous adenovirus expressing monomeric RFP (Ad-RFP) was used as control [44], [50], [51].
Red fluorescence protein gene (RFP) was also cloned into adenovirus vector as control (Ad-RFP).
The Red Fluorescent Protein (RFP) was used as a negative control, and the Ad-RFP was generated accordingly.
Ad-eGFP and Ad-RFP control adenoviruses were purchased from Vector Biolabs (Philadelphia, PA).
DOI: http://dx.doi.org/10.7554/eLife.00940.008 10.7554/eLiFigure40.009 Figure 3 figure supplement 1. Representative confocal images of spheres infected with control virus (Ad-RFP).
