Sentence examples for a reverse complement for from inspiring English sources

Before generating the database with DNA-BAR the sequences were run through a PERL script that added a reverse complement for each sequence in order to ensure that query sequences would match the database in either the forward or the reverse orientation.

Note that one should use a reverse complement for one of the aligned sequences to construct matrix S R together with maximizing the function in Equation (7).

Bowtie2 version 2.1.0 (settings: −-very-sensitive-local –N1) [ 73] and htseq-count script (http://www-huber.embl.de/users/anders/HTSeq/doc/overview.html) (using the option –s reverse, which reports reads mapping to annotated features on a reverse complement fashion) were used for this purpose.

This means that for genes from the complementary strand a reverse complement sequence has to be taken.

Each k-mer has a reverse complement.

For the analysis of sequencing data generated by reverse primers, we incorporated a reverse complement function along with its inferred chromatogram.

Reverse transcription was performed using a reverse complement DNA-oligonucleotide to the linker sequence.

In the other component it is applied in the negative direction as a reverse complement.

Using 236 oligonucleotides, their design designates a single oligonucleotide target and its reverse complement for each type of transposable element.

Therefore, we use a stringent cutoff of 50% sequence identity to ensure that the intergenic region had a significant inverted repeat above the background of the C. elegans genome (Figure S1 shows the distribution of percent identity to the reverse complement for all C. elegans intergenic regions).

We generated the reverse complement for those mRNAs transcribed from the negative strand.