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Thus, the tested target genes show a distinctive binding pattern of p53 phosphorylated at S46 upon Etoposide treatment which in some cases is also accompanied by a respective expression change.
We achieved nearly complete cloning of the first version of the Cpn ORFeome, and furthermore demonstrated the feasibility of a respective expression ORFeome in the form of recombinant protein-producing vectors and pooled Y2H prey vectors.
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We have used the RPKM measure for each gene as an estimation of their respective expression level.
Respective expression analyses showed a reduced expression of the ECM genes ACAN and COL2A1 (figure 4J), although the downregulation of COL2A1 appeared not statistically significant.
In order to further explore the associations between PXC1/2/3 and PXY/ TDR, we examined their respective expression patterns using a native promoter-driven GUS (β-glucuronidase) reporter system.
Of particular interest is the close clustering of hESC-ECs and HUVECs, indicating a high degree of similarity between their respective expression profiles.
Though hESC-ECs and HUVECs revealed a high degree of similarity between their respective expression profiles, hESC-ECs do not resemble HUVEC completely.
Both psoriasin and RanBPM were cloned by PCR into their respective expression vectors downstream of a T7 promoter and S-Met labeled proteins were generated.
Hence, the observed phenotypes in clutches 3 and on can be attributed to a complete absence of a respective gene expression.
Plasmids carrying genes of these EvpC mutants were used to transform an E. tarda ΔevpC mutant and their respective expression and secretion of EvpC were anlaysed.
In a subsequent study, however, they concluded that the respective expression levels of HLA-DR and CIITA were unrelated.
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