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Thus, although prions propagated efficiently in cells expressing individual Ssa proteins, freezing and thawing revealed a requirement for specific Hsp70 function to maintain prion stability under certain conditions.
This may be due to an improper folding of in vitro translated Woc or a requirement for specific posttranslational modifications that do not occur during in vitro translation and bacterial expression.
So far, a requirement for specific organelles for cephalosporin production in this filamentous fungus is unknown.
A distinct bias in nucleotide distribution post-selection/screening is indicative of a requirement for specific nucleotides for promoter function.
Comparison of I with III clearly shows that there is not a requirement for specific substrate geometry as long as Se is present in the scissile bond.
Furthermore, it was found that restimulation with anti-CD3 antibodies was essential for target cell lysis, indicating a requirement for specific T cell receptor stimulation.
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The implementation of HPV screening and increased coverage of HPV vaccination will lower the background risk of CIN3 and cancer, leading to longer screening intervals and a requirement for more specific tests 9. Therefore, type-specific serial measurements with a more sensitive and more specific HPV assay would allow earlier detection and treatment 7, 10.
To our knowledge, this is the first demonstration of a requirement for a specific extracellular matrix heparan sulfate proteoglycan in triglyceride metabolism in vivo.
We have included a requirement for a specific minimal amount of exposure to head impacts.
This would indicate a requirement for a specific mitochondrial deubiquitination enzyme (DUB) for DRP1, which has not yet been identified.
The high conservation of these residues in the CHHC Zn-finger domains may reflect a requirement for a specific backbone conformation.
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