Kinetic constraints which bias the phase selection away from the energetically most favored structure are usually the result of a requirement for ordering, complex atomic rearrangement, or partitioning during crystallization.
These architectural changes occur in parallel to a build-up of Crb protein in the cytoplasm of ept mutant cells (Fig. 2A') due to a requirement for ESCRT complexes in vesicular trafficking of Crb [4], [5], [30].
The practice of overlapping activities is becoming a requirement for fast-tracking complex construction projects.
Here, we define a requirement for the INO80 complex, a SWI/SNF family chromatin remodeler, in ESC self-renewal, somatic cell reprogramming, and blastocyst development.
We benchmark SGPA by examining two fundamental biological phenotypes: first, we explore glucose repression, in which SGPA identifies a requirement for the Mediator complex and a role for the CDK8/kinase module in regulating transcription.
However, a requirement for the WASH complex in β2AR recycling had not been demonstrated.
This could be blocked by inhibition or absence of caspase-8 suggesting a requirement for the DISC complex.
Initial treatment failure with prosthetic infections increases the probability of a requirement for a more complex procedure (2-stage exchange) with a greater burden for the patients, and might therefore end in an inferior functional outcome.
A large number of the substrates contain regions that antagonize translocation, causing a requirement for the IcmS/IcmW complex to act as a putative secretion chaperone that binds these regions.
Moreover, targeted RNAi knockdown of microtubule-associated proteins demonstrated a requirement for the Lis-1 complex in border cells (Yang et al. 2012).
