Required volume of a test sample was put on a measuring plate.
Without dilution, the high-HF concentration would lead to a damage of the electrode and an inappropriate increase of the required volume of the standard solution.
Extremely uniform layers (volume error < 1%) are achieved by precisely controlling the selection and ejection of multi-size droplets of ink material on a substrate so that only the required volume of material is deposited.
At the acquisition time of 2 min, the required volume of sample was approximately 2 pmol, a value indicating a highly sensitive analysis.
However, if a pH-dependent dissolution profile is necessary, the required volume of the acidic acceptor fluid is too large, since T20 does not increase CC solubility in acidic conditions so dramatically.
Trichostatinn A was dissolved in PBS and diluted with the required volume of the medium, and cells were also treated at different concentrations (4, 10, and 50 nM) of MG-132 (Calbiochem, San Diego, CA, USA) inhibitor of proteasome, dissolved in DMSO and diluted with the required volume of medium for 8 12 h prior to harvest for further investigations.
We chose PWMs to summarize TFBSs because validated PWMs are available from several sources [3], [4], and because PWMs are suitable for de novo discovery as they provide a good tradeoff between binding site prediction accuracy and the required volume of training data needed [11].
The required volume of sonicated seed solution was made to a final volume of 50 μL with distilled water and added to 200 μL of the 62.6 μM peptide monomer solution to give a final monomer concentration of 50 μM.
The required volume of each pellet was taken to obtain a final sperm concentration of 50 × 10 ml-1 in 5 ml of each of three commercial freezing extenders: Gent® (Minitüb®, Abfullund Labortechnik GmbH & Co., Tiefenbach, Germany), InraFreeze® (IMV technologies, L'Aigle, France) and Botu-Crio® (BioTech, Botucatu, Sao Paulo, Brazil) (total number of treatments therefore = 9; see Figure 2).
