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A YopE defective background was chosen for this assay in order to avoid a repressing effect on pore formation and thereby influence of ExoS translocation efficiency [74].
Interestingly, the transcription factor DksA, which normally acts synergistically with ppGpp, showed a repressing effect on rsd expression, indicating the possibility of a differential regulation by DksA and ppGpp.
In general [3], [8], methylation on H3K4, H3K36, and H3K79 has an activating effect on gene expression [6], [9], whereas methylation of H3K9, H3K27, and H4K20 impose a repressing effect on transcription [10] [13].
Strongest induction occurs by using low concentrations (0.5 mM), whereas high concentrations lead to a repressing effect on xylanase expression [ 7].
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Therefore, xylose had a lower repressing effect on enzyme production than glucose.
These regulating proteins can have an activating or repressing effect on the expression of a gene.
Since HC appears to have an upregulating effect on Oas2 and Slc5a9 expression rather than a repressing effect, it is possible that these genes are not regulated by unliganded GR through precisely the same mechanism as Brca1.
We also tested the repressing effect on p14 ARF for the human-specific sites.
With respect to gene regulation, the Malaysian genome harbors alleles with both activating and repressing effects on iron metabolism, reflecting a complex genetic model that involves YAP5 and CCC1 as well as AFT1.
This transcriptional regulatory network is represented as a graph where vertices are genes and directed edges denote activating or repressing effects on transcription.
Thus, the activating effects of Xrn1 on the translationally activated mRNAs would be slightly higher than our estimates, while the repressing effects on the translationally repressed mRNAs would be slightly lower.
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