Seierstad, looking unflinchingly at the repressed condition of Afghan women, "is a sharp and often lyrical observer," our critic, Richard McGill Murphy, wrote.
To further confirm the differential sensitivity to MMS and HU in the P MET3-MMS22 strain in the repressed condition, we checked the viability of the strains during a 12-hr period of MMS or HU exposure.
But her account of living in the family of an English-speaking Kabul bookseller, whom she portrays as a liberal to the world and a tyrannical patriarch at home, also shocked Nordic audiences with its intimate look at the repressed condition of Afghan women.
To confirm these results, we looked at the promoter of a gene previously reported to be TAF-dependent, ACT1 [22], and accordingly saw a large signal under repressed conditions when this gene is more active, and a weaker signal under derepressed conditions when activity decreases (Fig. 1H, inset).
We describe here a new expression vector, pLAC11, which was designed to be more regulable and thus more tightly repressible when grown under repressed conditions.
We did not observe either Pol II or TFIIB at any promoter under repressed conditions, as compared to a negative control locus (data not shown).
Bmh1 and Bmh2 bind to Adr1 phosphorylated on S230 (Parua et al. 2010) and expression of ADH2 under repressed conditions is increased in a bmh1 bmh2 strain and even further increased in a reg1 bmh1 bmh2 strain.
In this communication, we report on the design of several genetic elements which resulted in more regulable and thus more tightly repressible protein expression system when grown under repressed conditions.
Adr1 is difficult to detect in repressed conditions, so we used strains containing a multi-copy plasmid with ADR1 under control of the constitutive ADH1 promoter.
Although a 6-fold higher SUC2 expression is observed in repressed conditions, significant Snf1 dephosphorylation still occurs in this strain upon addition of glucose.
The induction of hicA expression increased the frequency of bacteria surviving ciprofloxacin exposure, in an arabinose concentration-dependent manner, from approximately 10−5 (under hicA repressed conditions) to 10−3 (0.1%) of the bacterial population at the highest arabinose concentration.
