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A representative specimen is shown in Figure S2 in Additional file 1. Adipose-derived stem cells (ASC) (isolated as described previously by [ 18]) were isolated from subcutaneous fat tissue that was obtained from patients undergoing elective body-contouring procedures.
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Where multiple replicate specimens were available from a single patient, one representative specimen was selected at random.
The inocula (from each representative specimen) were propagated in Marc-145 cells (QIAGEN, Hilden, Germany) for the viral genomic RNA isolation.
CLC Sequence Viewer 6.5.2 was exerted to align sequences and the alignments for the representative specimens were represented in Fig. 3.
A representative specimen set is important as the presence of an alteration may be associated with a particular pathologic subset as for example in a recent study that reported a significant correlation between BAP1 point mutation and high grade ccRCC while PBRM1 point mutation was associated with low grade ccRCC.
In the laboratory, representative specimens were prepared and subjected to a variety of tests, including Atterberg limits, standard Proctor compaction, unconfined compressive strength, unconsolidated undrained triaxial compression, wetting-induced collapse, and pinhole dispersivity.
Representative specimens were inspected under the scanning electron microscope.
Representative specimens were illustrated in Table 1.
As examples, pictures of representative specimens are reported in Figure 2.
Additionally, representative specimens were prepared for evaluating the thickness of the adhesive layer (Figure 1).
Images of type specimens were examined when available; otherwise images of several representative specimens were used.
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CEO of Professional Science Editing for Scientists @ prosciediting.com