The phrase "a replication timing" is not correct in written English.
It should be "replication timing" without the article "a" as it refers to a concept rather than a countable noun.
Example: "The study focused on replication timing during the S phase of the cell cycle."
Alternatives: "the timing of replication" or "replication schedule."
Additionally, PCR marker chr6.1395C, which is located in a region with a replication timing ratio of 1.58, replicates at 5.4 hrs, as do primer sets A and B from that same island that have Woodfine replication ratios of 1.36 and 1.25.
Two features of a replication timing profile were originally considered to be characteristic of pluripotent cells [ 45].
These ratios were then processed as were processed the microarray intensity ratios to calculate Trep values and generate a replication timing profile.
There has been an attempt to determine whether pluripotency is associated with distinct features of a replication timing profile in a genomic context [ 45].
But for somatic cells, with a much longer S phase driven by a replication timing programme and active checkpoint responses to delay entry into S phase, this might not pose a potential threat.
Instead, what we observe is that the pattern of the plot changes dramatically with loci now showing a replication timing profile strikingly different from control with large scale late to early and early to mid transitions.
Post-mitotic re-establishment of 3D chromatin architecture occurs at the TDP, and developmental cues that change a replication-timing program are likely to act during this short time window [ 115].
To test whether these perturbations altered global replication dynamics, we compared it with a replication-timing experiment that used an unperturbed S phase and was performed in the same strain background (Müller and Nieduszynski, 2012).
A replication-timing program, which governs the transcriptional competence of chromosome domains, is established during early G1 phase, a short window of opportunity termed the timing decision point (TDP) [ 115].
Interestingly, in the second study, neocentromere formation caused sequences adjacent to the CENP-A domain to shift to a later replication timing.
In contrast, an early replicating domain shifted to a later replication timing following neocentromere formation within it.
