Exact(3)
Accordingly, the structure of the fluorescent signal of each identified region was noted: either singlet (S), denoting a non-replicated sequence, or doublet (D), disclosing a replicated sequence.
The structure of each signal, either singlet (S), representing a non-replicated sequence, or doublet (D), disclosing a replicated sequence, was noted.
Accordingly, following hybridization, the fluorescence signals are divided into two categories, a single dot-like signal (designated: "singlet" or "S"), representing a yet non-replicated DNA sequence and a duplicated bipartite signal (designated: "doublet" or "D"), indicating a replicated sequence.
Similar(57)
We identified a set of DE transcripts among root samples using high throughput replicated sequence data.
In addition, they contained a yeast centromere, an autonomously replicating sequence (ARS), positive and negative selectable markers as well as an ori and ampR gene for replication in E. coli cells.
They contain the yeast selectable marker URA3, the negative-selection marker CYH2 [40], a yeast centromere and an autonomously replicating sequence (ARS).
The organization of these forms and the identification of an autonomously replicating sequence functional in S. cerevisiae, strongly suggest that an extrachromosomal circular DNA (eccDNA) molecule serves as an intermediate in the amplification of the Z. bailii region in yeast genomes.
Plotting transcript fold changes levels shows a high correlation among the biologically replicated sequencing runs as indicated by Euclidean distances.
The artificially replicated sequences that were an artifact of the 454-based pyrosequencing technique were identified and eliminated using the Replicates software (Gomes-Alvarez et al. 2009).
Replicates [ 79] software was used to identify and eliminate the artificially replicated sequences produced during the 454-based pyrosequencing.
The replicated sequences matched those obtained in the Florence laboratory (see Table S2, see Additional file 2).
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